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ipvh-membrane 96-well plates  (Millipore)


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    Structured Review

    Millipore ipvh-membrane 96-well plates
    Ipvh Membrane 96 Well Plates, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ipvh-membrane+plates/96+well+filter+plate/pm36785938-42-18-21
    Average 90 stars, based on 1 article reviews
    ipvh-membrane 96-well plates - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Mouse Assay:

    Article Title: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
    Article Snippet: For analysis of IFN-γ production by ELISpot assay, splenocytes were stimulated with S peptide pools at a final concentration of 2 μg/mL on IPVH-membrane plates (Millipore) coated with 5 μg/mL anti-mouse IFN-γ (clone AN18; Mabtech).

    Article Title: Immunogenic composition and use thereof
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Article Title: 4-1BBL Enhances CD8 + T Cell Responses Induced by Vectored Vaccines in Mice but Fails to Improve Immunogenicity in Rhesus Macaques
    Article Snippet: For detection of murine antigen specific IFN-γ producing cells, ammonium chloride-potassium (ACK) lysis buffer treated mouse splenocytes or PBMCs were stimulated with the relevant peptide (final concentration 1–2 μg/ml) for 18–20 hours on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Adenovirus vectors
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI; SEQ ID NO: 12) at a final concentration of 1 μg/ml.

    Article Title: ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
    Article Snippet: Mice – For analysis of IFN-γ production by ELISpot, cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18).

    Article Title: STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines
    Article Snippet: In brief, single cell suspensions of splenocytes were treated with ammonium chloride-potassium (ACK) lysis buffer and cells plated on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Simian adenoviral vectors encoding malaria antigen
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Enzyme-linked Immunospot:

    Article Title: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
    Article Snippet: For analysis of IFN-γ production by ELISpot assay, splenocytes were stimulated with S peptide pools at a final concentration of 2 μg/mL on IPVH-membrane plates (Millipore) coated with 5 μg/mL anti-mouse IFN-γ (clone AN18; Mabtech).

    Article Title: Immunogenic composition and use thereof
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Article Title: 4-1BBL Enhances CD8 + T Cell Responses Induced by Vectored Vaccines in Mice but Fails to Improve Immunogenicity in Rhesus Macaques
    Article Snippet: For detection of murine antigen specific IFN-γ producing cells, ammonium chloride-potassium (ACK) lysis buffer treated mouse splenocytes or PBMCs were stimulated with the relevant peptide (final concentration 1–2 μg/ml) for 18–20 hours on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Adenovirus vectors
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI; SEQ ID NO: 12) at a final concentration of 1 μg/ml.

    Article Title: ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
    Article Snippet: Mice – For analysis of IFN-γ production by ELISpot, cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18).

    Article Title: STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines
    Article Snippet: In brief, single cell suspensions of splenocytes were treated with ammonium chloride-potassium (ACK) lysis buffer and cells plated on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Simian adenoviral vectors encoding malaria antigen
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Concentration Assay:

    Article Title: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
    Article Snippet: For analysis of IFN-γ production by ELISpot assay, splenocytes were stimulated with S peptide pools at a final concentration of 2 μg/mL on IPVH-membrane plates (Millipore) coated with 5 μg/mL anti-mouse IFN-γ (clone AN18; Mabtech).

    Article Title: Immunogenic composition and use thereof
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Article Title: 4-1BBL Enhances CD8 + T Cell Responses Induced by Vectored Vaccines in Mice but Fails to Improve Immunogenicity in Rhesus Macaques
    Article Snippet: For detection of murine antigen specific IFN-γ producing cells, ammonium chloride-potassium (ACK) lysis buffer treated mouse splenocytes or PBMCs were stimulated with the relevant peptide (final concentration 1–2 μg/ml) for 18–20 hours on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Adenovirus vectors
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI; SEQ ID NO: 12) at a final concentration of 1 μg/ml.

    Article Title: ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
    Article Snippet: Mice – For analysis of IFN-γ production by ELISpot, cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18).

    Article Title: STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines
    Article Snippet: In brief, single cell suspensions of splenocytes were treated with ammonium chloride-potassium (ACK) lysis buffer and cells plated on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Simian adenoviral vectors encoding malaria antigen
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Ex Vivo:

    Article Title: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
    Article Snippet: For analysis of IFN-γ production by ELISpot assay, splenocytes were stimulated with S peptide pools at a final concentration of 2 μg/mL on IPVH-membrane plates (Millipore) coated with 5 μg/mL anti-mouse IFN-γ (clone AN18; Mabtech).

    Article Title: Immunogenic composition and use thereof
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Article Title: 4-1BBL Enhances CD8 + T Cell Responses Induced by Vectored Vaccines in Mice but Fails to Improve Immunogenicity in Rhesus Macaques
    Article Snippet: For detection of murine antigen specific IFN-γ producing cells, ammonium chloride-potassium (ACK) lysis buffer treated mouse splenocytes or PBMCs were stimulated with the relevant peptide (final concentration 1–2 μg/ml) for 18–20 hours on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Adenovirus vectors
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI; SEQ ID NO: 12) at a final concentration of 1 μg/ml.

    Article Title: ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
    Article Snippet: Mice – For analysis of IFN-γ production by ELISpot, cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18).

    Article Title: STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines
    Article Snippet: In brief, single cell suspensions of splenocytes were treated with ammonium chloride-potassium (ACK) lysis buffer and cells plated on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Simian adenoviral vectors encoding malaria antigen
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Cell Culture:

    Article Title: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
    Article Snippet: For analysis of IFN-γ production by ELISpot assay, splenocytes were stimulated with S peptide pools at a final concentration of 2 μg/mL on IPVH-membrane plates (Millipore) coated with 5 μg/mL anti-mouse IFN-γ (clone AN18; Mabtech).

    Article Title: Immunogenic composition and use thereof
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Article Title: 4-1BBL Enhances CD8 + T Cell Responses Induced by Vectored Vaccines in Mice but Fails to Improve Immunogenicity in Rhesus Macaques
    Article Snippet: For detection of murine antigen specific IFN-γ producing cells, ammonium chloride-potassium (ACK) lysis buffer treated mouse splenocytes or PBMCs were stimulated with the relevant peptide (final concentration 1–2 μg/ml) for 18–20 hours on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Adenovirus vectors
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI; SEQ ID NO: 12) at a final concentration of 1 μg/ml.

    Article Title: ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
    Article Snippet: Mice – For analysis of IFN-γ production by ELISpot, cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18).

    Article Title: STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines
    Article Snippet: In brief, single cell suspensions of splenocytes were treated with ammonium chloride-potassium (ACK) lysis buffer and cells plated on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Simian adenoviral vectors encoding malaria antigen
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Lysis:

    Article Title: Evaluation of the immunogenicity of prime-boost vaccination with the replication-deficient viral vectored COVID-19 vaccine candidate ChAdOx1 nCoV-19
    Article Snippet: For analysis of IFN-γ production by ELISpot assay, splenocytes were stimulated with S peptide pools at a final concentration of 2 μg/mL on IPVH-membrane plates (Millipore) coated with 5 μg/mL anti-mouse IFN-γ (clone AN18; Mabtech).

    Article Title: Immunogenic composition and use thereof
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.

    Article Title: 4-1BBL Enhances CD8 + T Cell Responses Induced by Vectored Vaccines in Mice but Fails to Improve Immunogenicity in Rhesus Macaques
    Article Snippet: For detection of murine antigen specific IFN-γ producing cells, ammonium chloride-potassium (ACK) lysis buffer treated mouse splenocytes or PBMCs were stimulated with the relevant peptide (final concentration 1–2 μg/ml) for 18–20 hours on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Adenovirus vectors
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI; SEQ ID NO: 12) at a final concentration of 1 μg/ml.

    Article Title: ChAdOx1 nCoV-19 vaccination prevents SARS-CoV-2 pneumonia in rhesus macaques
    Article Snippet: Mice – For analysis of IFN-γ production by ELISpot, cells were stimulated with pools of S1 or S2 peptides (final concentration of 2μg/ml) on IPVH-membrane plates (Millipore) coated with 5μg/ml anti-mouse IFN-γ (AN18).

    Article Title: STING-pathway modulation to enhance the immunogenicity of adenoviral-vectored vaccines
    Article Snippet: In brief, single cell suspensions of splenocytes were treated with ammonium chloride-potassium (ACK) lysis buffer and cells plated on IPVH-membrane plates (Millipore) coated with 5 μg/ml anti-mouse IFN-γ (AN18) (Mabtech).

    Article Title: Simian adenoviral vectors encoding malaria antigen
    Article Snippet: Ex Vivo IFNγ ELISPOT ACK-treated splenocytes or PBMCs were cultured for 18-20 hours on IPVH-membrane plates (Millipore) with the immunodominant H-2Kd-restricted epitope Pb9 (SYIPSAEKI) at a final concentration of 1 μg/ml.



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